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Download Edraw Max Premium 2016 with Crack for Mac OSStriatal dopamine (DA) depletion by 6-hydroxydopamine in rats: behavioral effects.
The behavioral changes produced in rats by bilateral striatal DA lesions were assessed on the basis of the hypoactivity previously reported after 6-hydroxydopamine (6-OHDA) treatment. The lesion was made with a neurotoxin which does not penetrate the blood-brain barrier and which therefore kills only the DA neurons in the ipsilateral striatum. This lesion was produced by the chronic intracerebral infusion of 8 micrograms of 6-OHDA into each striatum. Twelve days later, all animals showed a marked decrease of the turn- and walkway-right activities in the open field and an increase of the tail-flicks and head-shapes frequencies. In the hole-board test, a decrease of the exploratory activity (a lower number of entries) was observed when compared to control or to animals given the same dose of 6-OHDA and the saline vehicle. Despite the motor impairment, which was assessed by the rearing behavior, most rats did not show any impairment in the rotarod test. In addition, the reversal of the lesion was analyzed by measuring striatal DA and DOPAC (DA and 3,4-dihydroxyphenylacetic acid, respectively) levels after striatal 6-OHDA lesions. Seventy percent of DA and 60% of DOPAC were depleted after the lesion. A partial restoration of DA levels was observed 24 days after the surgery. The present results showed that, despite the severe DA lesion, only motor impairment was observed after the 6-OHDA treatment. Furthermore, no increase of motor activity was observed after the removal of the DA decrease.Q:
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