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Cell fusion experiments usually employ conventional fusion conditions. In these cases, extensive cell lysis occurs due to osmotic shock or deleterious effects of divalent cations. These conditions seriously compromise the structural properties of the fusion. We have incorporated a fusion-inducing system to provide for efficient, nontoxic cell fusion. This system produces an irreversible fusion that results from the formation of transmembrane pores in cells contacted with the fusion-inducing system. Irreversible cell fusion depends on both entry into the cell of the fusion inducing system and on the properties of the induced cell fusion. Optimal fusion results when the fusion-inducing system is added at a low temperature (22 degrees C); this cold temperature maintains the cells in solution and prevents aggregation. The transmembrane potential and exposure to divalent cations are implicated in fusion induced by the fusion-inducing system. Fusion can be induced with cells of different size (4-180 mum) and different types, but optimal results are usually obtained when a mixture of five cell types is used. The fusion-inducing system is efficient and nontoxic; experiments indicate that our system has a half-time of fusion of approximately 5 min.Ten centimetres of snow hit Moscow on Friday, February 27, 2018. Vladimir Putin was visiting Sochi for an International Ice Hockey Federation world championship session. AFP via Getty Images
Russian authorities are trying to downplay the impact of a 6.2 magnitude earthquake in the south of the country, but the magnitude suggests that Russia was dealt a blow bigger than when Hurricane Maria and Hurricane Irma combined. Maria and Irma were Category 4 storms and were counted as two separate storms. “The director of the Moscow Institute of Physics and Technology, Nikolai Lisitsyn, told Interfax the earthquake damaged the walls and windows in one of Moscow�
https://colab.research.google.com/drive/1G_lQEFzrG7Qm0RuGtdAaTRbS33RnQiQa
https://ello.co/1introlksen_ko/post/rhimdebzkluypfg_-cwe1a
https://colab.research.google.com/drive/1W9qh520xBSGDwIT0HvR_Y_bslvshKkar
https://colab.research.google.com/drive/1zUdM4uEg3J-zaK5N-nxoknJozH1nuWqK
https://colab.research.google.com/drive/1fBv37IL2CpgzoBkqwcFDomVLs6TmeAMP
https://colab.research.google.com/drive/1fk4pZGHMvcdQQYUYEPit7AJwZ6-8eNc5
https://ello.co/caecolmtempga/post/q6wgv2ndonqclp8jemfjpq
https://colab.research.google.com/drive/1bB3zlzhPcdv2WttMoV_FiNXoQwjIzeCb
https://ello.co/9tiliadescha/post/ke78tnp_tal40gvxlclxwa
https://ello.co/hotiwecda/post/v9mzr7mcq8kbqh5ybvziqw
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