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Download X6-7V 7in-Netbook WM8650 CE6.0.rar.A membrane fraction of the kidney cortex mediates the increase of renal blood flow induced by the cyclic nucleotide phosphodiesterase inhibitor, papaverine.
The role of the membrane fraction (M1) of the rat kidney cortex has been studied in the modulation of the glomerular filtration rate (GFR) and renal blood flow (RBF) induced by the cyclic nucleotide phosphodiesterase inhibitor, papaverine. Papaverine increased both GFR and RBF in bilaterally nephrectomized rats, which were treated in the last 24 h with the same regimen of papaverine and vehicle. The increase in RBF by papaverine, 48 h after the treatment, was abolished when M1 was added to the perfusate of the isolated rat kidney. The addition of papaverine to the perfusate of the isolated kidney increased RBF only if the perfusate contained the M1 fraction isolated from the homologous kidney. Thus, M1 is a target of papaverine action. Under basal conditions, 12% of the total papaverine dose administered to the isolated perfused kidney was recovered in the perfusate indicating that the kidney does not metabolize the phosphodiesterase inhibitor. The addition of papaverine to the perfusate of the isolated kidney increased the levels of cAMP and cGMP in the renal tissue. The addition of M1 to the perfusate significantly lowered the levels of cAMP in the renal tissue under both basal and papaverine-stimulated conditions. These results indicate that M1 isolated from the renal tissue is involved in the mediation of papaverine action in the kidney.Q:
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