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Technologies that have focused on monitoring the activation of highly purified T cells have been instrumental to defining the nature of antigen-specific T cell responses. However, many investigators would benefit from having available a solid phase capture assay in which the limiting aspects of T cell stimulation could be evaluated. Here we show that preparations of anti-idiotype antibody, immobilized on polystyrene plate wells, are highly efficient at stimulating antigen-specific B cells in culture. In a direct comparison, stimulation by immobilized anti-idiotype antibody was significantly more effective than immobilized anti-idiotype monoclonal antibody, which requires multiple cross-linking on plastic surfaces to support B cell activation. Stimulation by immobilized anti-idiotype antibody was also superior to the activation of T cells by immobilized antibodies and was effective in the presence of high concentrations of T cells. To investigate the mechanism of this response, we found that the

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