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A systematic investigation of factors affecting the derivatization of azo dyes in fish tissues, using variable solvent composition for ultrasonic-assisted extraction.
The derivatization process of azo dyes in fish tissues was systematically investigated using a common solvent [methanol-2-propanol-HCl mixture (80:10:10, v/v)] for ultrasonic-assisted extraction. The factors including types of dyes, organic solvents, extraction time, sonication time and temperature were examined and optimized. The main factors affecting the derivatization yield were the types of organic solvents, extraction time and sonication time. The optimal conditions were as follows: the concentration of NaCl in the extraction solvent was 0.65% (w/v), the extraction time was 60 min and the sonication time was 8 min. The average derivatization yields for 15 types of dyes studied using this optimal condition were 61.0% on an average. Although this condition was not suitable for all types of dyes, the derivatization conditions could be useful for the practical utilization of azo dyes in fish tissues.BCL11B
B-cell CLL/lymphoma 11B is a protein that in humans is encoded by the BCL11B gene.
Function
This gene is a member of the MECOM family, and encodes a zinc finger transcription factor. The encoded protein forms a complex with MYBL2 and synergistically represses transcription. This protein has been shown to inhibit CD19-mediated B cell differentiation through the direct repression of MYB and other B cell-specific genes. Alternate transcriptional splice variants have been characterized.
Interactions
BCL11B has been shown to interact with:
CREB1,
EP300,
MYBL2,
Nuclear receptor coactivator complex
Nuclear receptor coactivator 1, and
TWIST1.
Clinical significance
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