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Docetaxel (DTX)-based nanomicelles, a new type of nanomicelles with improved antitumor efficacy, were constructed to develop a synergistic combination of docetaxel and doxorubicin (DOX) with curcumin (CUR). In this study, novel DTX-based nanomicelles, with a diameter less than 50 nm, were successfully prepared, and their surface morphology, microstructure, drug loading content and in vitro release kinetics of DOX and CUR were investigated. Results showed that the doxorubicin and curcumin had good self-assembly ability, and DTX-based micelles formed a small hydrophobic core to confine drugs and at the same time acted as a matrix to protect the encapsulated drugs. The synergistic effects of DTX, DOX, and CUR were evaluated by using CalcuSyn software. The CUR inhibition efficacy of the DTX-based nanomicelles was higher than free CUR, and the inhibition efficacy of the DTX-based nanomicelles was higher than that of the free DOX, which was in agreement with the premise that the combination of CUR and DOX was more advantageous than CUR alone. The median survival times of the nude mice bearing A549 xenograft tumors treated with DTX, DOX, CUR, and DTX-based nanomicelles were 33.5, 45.4, 58.2, and 91.0 days, respectively. The results of this study indicated that the DTX-based nanomicelles
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