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The use of amine oxidase coupled with peroxidase (AO-PO) for the amperometric detection of tyramine in food samples is a well-known technique. The kinetic and mathematical modeling of AO-PO activity, however, remains to be fully developed. In this paper, a method for the simultaneous determinations of kinetic parameters associated with amine oxidase (from bovine plasma) and peroxidase (from horseradish root) coupled activities on different substrates was developed. The Michaelis-Menten parameters K(M) and V(max) could be calculated by applying the experimental data obtained from specific measurements on the AO activity. On the other hand, the V(max) values obtained for the AO-PO reaction were firstly normalized to the values obtained for the hydrogen peroxide (H(2)O(2)) production, and then the Michaelis-Menten constants for tyramine oxidized by AO-PO reaction were calculated. A kinetic equation was built by using specific values of both K(M) and V(max) calculations, which gave a good estimation of the experimental data. In this study, tyramine was first oxidized by the AO-PO system and then the oxidation of aminophenols was measured. The kinetic modeling of the AO-PO reaction was presented and could be a simple and useful tool for the electrochemical determination of aminophenols.Q:
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Des Moines Mayor Frank Cownie hailed the City Council’s unanimous vote late Friday to approve the apartment complex planned for a vacant lot in the West Des Moines historic district.
The nine-story building would be the largest in the city, with almost 500 units. The complex would include retail space on the ground floor.
Des Moines Councilor Ryan Drabek represented the neighborhood on the council and was the driving force behind the plan to restore the historic house at the site, 1402 Skutnik Drive.
Drabek said the apartment building is the culmination of years of effort to redevelop the block.
“I am thrilled with the council’s decision,” Drabek said. “It is a testament to the hard work of so many. It is a heavy lift, but at the end of the day we have gotten to a place where now we can start building.”
The site is the last remaining house on the block. It is not considered by preservationists to be as historic as the Elms cottage. It was
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